Description:
Animal Total RNA Isolation kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples.
The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.
Features&advantages:
-No need to worry about RNA degradation; the whole system is RNase-Free
-Effectively remove DNA-using DNA-Cleaning Column
-Remove DNA without adding DNase
-Simple-all operations are completed at room temperature
-Fast -operation can be completed in 30 minutes
-Safe-no organic reagent extraction required
-High purity -OD260/280≈1.8-2.1
Kit application:
It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.
Product Parameters:
-Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
-Samples: animal tissues, cultured cells
-Dosage: Tissues 10-20mg, Cells(2-5)×106
-Maximum DNA binding capacity of purification column: 80 μg
-Elution volume: 50-200 μl
Work Flow:
Diagram:
Storage and Shelf life:
The kit can be stored for 24 months at room temperature (15–25 ℃) and 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).
