Description:
This kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from cells cultured in 96, 24, 12, and 6-well plates. The kit provides an efficient DNA-Cleaning Column, which can easily separate the supernatant and cell lysate, bind and remove genomic DNA. The operation is simple and time-saving; the RNA-only Column can efficiently bind RNA with a unique formula. A large number of samples can be processed simultaneously.
The whole system is RNase-Free, so that the purified RNA is not degraded; the Buffer RW1, Buffer RW2 buffer washing system guaratee the obtained RNA free of protein, DNA, ion, and organic compound pollution.
Features&advantages:
-The whole process is operated at room temperature (15-25℃), without ice bath and low temperature centrifugation.
-The whole kit is RNase-Free, no need to worry about RNA degradation.
-DNA-Cleaning Column specifically binds DNA, so that the kit can remove genomic DNA contamination without adding additional DNase.
-High RNA yield: RNA-only Column and unique formula can efficiently purify RNA.
-Fast speed: easy to operate and can be completed in 11 minutes.
-Safety: No organic reagent is required.
-High quality: The purified RNA is of high purity, free of protein and other impurities, and can meet various subsequent experiments.
Kit application:
It is suitable for extraction and purification of total RNA from cultured cells in 96, 24, 12, and 6-well plates.
Work Flow:
Diagram:
The agarose gel battery diagram of Cell Total RNA Isolation Kit treated the above different numbers of cells,20μl volume elution, take 2μl purified total RNA 1%.
Storage and Shelf life:
The kit can be stored for 12 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time(24 months).
Buffer cRL1 can be stored at 4 ℃ for 1 month after adding 2-hydroxy-1-ethanethiol(optional).
