Description:
This kit provides a quick and easy method to extract genomic DNA from bacteria and food residues from stool samples from various sources. There are a large number of inhibitors in stool samples. Even if these substances are present in a small amount in the purified DNA, they will affect downstream reactions, such as PCR and restriction enzyme digestion. Therefore, the key to purification of fecal DNA is how to effectively remove inhibitors in feces.
This kit uses DNA-Only Column that can specifically bind DNA, a brand-new Foregene Protease and a unique buffer system, which can effectively remove various inhibitors in feces, without organic solvent extraction or ethanol and isopropanol precipitation. The DNA extraction operation from stool samples can be completed in 40 minutes.
Features&advantages:
-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA from the genomic DNA without adding RNase during the experiment, preventing the laboratory from being contaminated by exogenous RNase.
-Fast speed: The operation is simple, and the fecal genomic DNA extraction operation can be completed within 40 minutes.
-Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
-Safety: No organic reagent extraction is required.
-High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Kit parameters:
Kit Application:
It is suitable for purification of genomic DNA from the following samples: fresh or frozen human, cow, chicken, mouse and other fecal samples.
Genomic DNA Appliaction:
The genomic DNA purified by Stool DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.
Work Flow:
Storage and Shelf life:
-This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
