Description:
This kit uses a unique lysis buffer system that can quickly release RNA from cultured cell samples for RT-qPCR reactions, thereby eliminating the time-consuming and laborious RNA purification process. The RNA template can be obtained in just 7 minutes. The 5×Direct RT Mix and 2×Direct qPCR Mix-SYBR reagents provided by the kit can quickly and effectively obtain real-time quantitative PCR results.
5×Direct RT Mix and 2×Direct qPCR Mix-SYBR have strong inhibitor tolerance, and the lysate of the samples can be used as the template for RT-qPCR directly. This kit contains the unique RNA high-affinity Foregene reverse transcriptase, and Hot D-Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR optimizer and stabilizer.
Specifications:
200×20μl Rxns, 1000×20μl Rxns
Product components:
Part I | Buffer CL |
Foregene Protease Plus II |
Buffer ST |
Part II | DNA Eraser |
5× Direct RT Mix |
2× Direct qPCR Mix-SYBR |
50× ROX Reference Dye |
RNase-Free ddH2O |
Instructions |
Features&advantages:
-Simple and effective : with Cell Direct RT technology, RNA samples can be obtained in just 7 minutes.
-The sample demand is small, as low as 10 cells can be tested.
-High throughput: it can quickly detect RNA in cells cultured in 384, 96, 24, 12, 6-well plates.
-DNA Eraser can quickly remove released genomes, greatly reduce the impact on subsequent experimental results.
-Optimized RT and qPCR system makes the two-step RT-PCR reverse transcription more efficient and PCR more specific, and more resistant to RT-qPCR reaction inhibitors.
Kit application:
-Scope of application: cultured cells.
-RNA released by sample lysis: only applicable to the RT-qPCR template of this kit.
-The kit can be used for the following purposes: gene expression analysis, verification of siRNA-mediated gene silencing effect, drug screening, etc.
Diagram:
Storage and Shelf life:
Part I of this kit should be stored at 4℃; Part II should be stored at -20℃.
vForegene Protease Plus II should be stored at 4℃,do not freeze at -20℃.
vReagent 2×Direct qPCR Mix-SYBR should be stored at -20℃ in the dark; if used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).
