Description
The 2× Real PCR EasyTM Mix-Taqman provided by the Real Time PCR EasyTM-Taqman kit is a new premix system that uses specific fluorescent probes for Real Time PCR amplification reactions, which can greatly improve product specificity and reaction sensitivity. At the same time, ROX is provided as an internal control dye. The Real Time PCR Mix has high amplification efficiency and can more sensitively and intuitively react the concentration of target template DNA.
2× Real PCR EasyTM Mix-Taqman contains our company’s unique hot-start Foregene Taq DNA Polymerase, which has the advantages of high amplification efficiency, strong specific amplification ability, and low mismatch rate compared with ordinary Taq enzymes. Used for fluorescent quantitative PCR reaction can reduce non-specific amplification and improve the accuracy of PCR.
Features
u Unique PCR optimization system makes 2× Real PCR EasyTM Mix-Taqman more compatible.
u Hot-start Foregene Taq Polymerase has higher amplification efficiency, higher amplification sensitivity, and higher amplification specificity.
u 2× Real PCR EasyTM Mix-Taqman uses a unique system optimized by Foregene to improve the sensitivity and specificity of sequence-specific probe detection.
u This product comes with ROX internal reference dye, which can be used to eliminate signal background and signal errors between wells, which is convenient for customers to use with different models of quantitative PCR instruments.
Kit application
Fluorescence quantitative PCR for template quantitative analysis/conventional PCR amplification/can be used for allele detection.
Product quality control
According to FOREGENE's Total Quality Management System, each batch of Real Time PCR EasyTM-Taqman is strictly tested for multiple times to ensure the reliability and stability of the quality of each batch of kits.
Kit contents
Real Time PCR EasyTM-Taqman (20μl system) |
Kit composition | QP-01021 | QP-01022 | QP-01023 | QP-01024 |
200 Preps | 500 Preps | 1000 Preps | 2000 Preps |
2× Real PCR EasyTM Mix-Taqman | 1ml×2 | 1.7ml×3 | 1.7ml×6 | 1.7ml×12 |
20× ROX Reference Dye | 200μl | 0.5ml | 1ml | 1ml×2 |
DNase-Free ddH2O | 1.7ml | 1.7ml×2 | 10ml | 20ml |
Manual | 1 | 1 | 1 | 1 |
Storage conditions
1. Transportation conditions
The whole process of low-temperature ice box transportation, to ensure that the kit is in a state of <4 ℃.
2. Storage conditions
The kit is stored at -20°C in the dark; if used frequently, it can also be stored at 4°C for short-term storage (use up within 10 days).
Table 1: PCR reaction system preparation
PCR system additions | Consumption | Final concentration |
2× Real PCR EasyTM Mix-Taqman | 10μl | 1× |
Forward Primer(10μM) | 0.8μl | 50-900nM 1* |
Reverse Primer(10μM) | 0.8μl | 50-900nM 1* |
Probe(4μM) | 1μl | 200nM |
Template (DNA) | X μl | 2* |
20× ROX Reference Dye | - | 3* |
DNase-Free ddH2O | (7.4-X) μl |
|
Total Volume | 20μl |
|
1*:Generally, a final primer concentration of 400 nM can get better results. When the reaction performance is poor, the primer concentration can be adjusted in the range of 50-900nM.
2*:The amount of DNA template added is usually below 100ng. Because different types of DNA templates contain different copy numbers of target genes, gradient dilution can be performed if necessary to determine the optimal DNA template addition amount.
3*:Choose the appropriate final concentration of ROX Reference Dye according to different quantitative PCR instruments. The optimal concentration of ROX Reference Dye for common quantitative PCR machines is shown in the following table:
