Description:
The kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality total RNA from various plant tissues with high polysaccharides or polyphenols content. It provides the DNA-Cleaning column that can easily remove genomic DNA from the supernatant and tissue lysate. RNA-only column can effectively bind RNA. The kit can process a large number of samples at the same time.
The entire system does not contain RNase, so the purified RNA will not be degraded. Buffer PRW1 and Buffer PRW2 can ensure that the RNA obtained is not contaminated by protein, DNA, ions, and organic compounds.
Features&advantages:
-Particularly suitable for the purification of RNA from plant samples with high polysaccharides and polyphenols content.
-No need to worry about RNA degradation. The whole system is RNase-Free
-Effectively remove DNA using DNA-Cleaning Column
-Remove DNA without adding DNase
-Simple—all operations are completed at room temperature
-Fast—operation can be completed in 30 minutes
-Safe—no organic reagent used
-High quality—the purified RNA is highly pure, free of protein and other impurities, and can meet various downstream experimental applications.
Product parameters:
-Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
Sample: Fresh or frozen plant tissues with high polysaccharides or polyphenols content.
- Sample size: 50mg plant tissue
-Maximum RNA binding capacity of purification column: 80 μg
-Elution volume: 50-200 μl
Kit application:
It is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with high polysaccharide and polyphenol content.
Work Flow:
Diagram:
Plant Total RNA Isolation Plus Kit was used to treat 20mg fresh leaves with high polysaccharide or polyphenol content. 5% of purified total RNA was used to run 1% agarose gel electrophoresis
1: Banana, 2: Ginkgo, 3: Cotton, 4: Pomegranate
Storage and Shelf life:
The kit can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time. Buffer PSL1 can be stored at 4℃ for 1 month after adding β-mercaptoethanol (it is recommended to add it at the same time as the experiment).
